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KMID : 0364219910340020196
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Korean Journal of Zoology
1991 Volume.34 No. 2 p.196 ~ p.202
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Identification and Isolation of Juvenile Hormone Binding Protein from Hemolyrnph of Lymantria dispar L.
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Lee In-Hee
Kim Hak-Ryul
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Abstract
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Juvenile honnone binding protein (JHBP) was identified in the last instar larval hemolymph of Lymantria dispar using dextran coated charcoal (DCC) binding assay and gel filtration. The p1 value of JHBP was estimated to be 5.3. JHBP was partially pudfied by polyethylene glycol(PEG) precipitation, DEAE-cellulose ion-exchange chromatography and gel filtration, and was confirmed by DCC binding assay.
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KEYWORD
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JHBP, Hemolymph, Lymantria dispar
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